Fig 1: ELISA results of VTN and COL1A1 between VUR and non-VUR groups. (A) ELISA result of VTN between VUR and non-VUR groups. (B) ELISA result of COL1A1 between VUR and non-VUR groups. (C) Receiver operating curve analysis of VTN between VUR and non-VUR groups. n=25 for the VUR group and n=35 for the non-VUR group. ****P<0.0001. n.s., not significant; VUR, vesicoureteral reflux; COL1A1, a-1 type I collagen; VTN, vitronectin; AUC, area under curve.
Fig 2: Western blotting detection of VTN and COL1A1 between VUR and non-VUR groups. (A) Western blotting results from similar loaded amounts of proteins stained by Coomassie G250 methods. (B) Western blotting results of VTN and COL1A1 when similar amounts of proteins were loaded between VUR and non-VUR groups. (C) VTN/total protein intensity ratio between VUR and non-VUR groups. (D) COL1A1/total protein intensity ratio between VUR and non-VUR groups. n=10 for each group. ***P<0.001, *P<0.05. VUR, vesicoureteral reflux; COL1A1, a-1 type I collagen; VTN, vitronectin.
Fig 3: Hypoxia-induced ADSC-Exos inhibited fibrous and degenerated cartilage in IL-1β-induced OA-like articular chondrocytes. (A) Fibrous cartilage genes, col1a1, and (B) degeneration marker gene, col10a1, mRNA expression in vitro in HACs treated with IL1-β and with or without ADSC-Exos (109 particles/mL) for 12 days. GAPDH mRNA was used as the internal control. (C) Type I and (D) type X collagen ELISAs were performed to quantify the protein levels when HACs were treated with IL1-β and with or without ADSC-Exos (107~109 particles/mL) for 12 days. Each column represents the mean ± SEM of six replicate cultures. Data from each group were compared with those of the IL-1β only group, *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Supplier Page from ABclonal Technology for Human Collagen Type I Alpha 1 ELISA Kit (COL1a1)